#Remyelination in the CNS is influenced by myeloid cells, including resident #microglia & infiltrating #monocytes. This study shows that infiltrating monocytes inhibit the efficiency of CNS remyelination @PLOSBiology https://plos.io/3RryCLF
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#Monocytes
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Human aging is assoc. with inflammation and intestinal barrier dysfunction. One might assume that barrier permeability would spark increased inflammation. This (and prev.) work suggests the opposite—that #inflammaging, driven by #monocytes and #macrophages appear to *cause* the barrier dysfunction!
Question to anyone familar with #TransHealthcare #Hematology
Is there a relationship between elevated #WBC (#neutrophils, #lymphocytes, #monocytes) & #Platelets, decreased #RBC, #Hemocrit, #Anemia, #Hgb, #mpv and #HRT for male to female #transgender individuals with no other evidence of blood disease, infection or #cancer?
#CTScans are clean, #BoneMarrowBiopsy clean. Doc wants to repeat however.
Infiltrating #monocytes drive #cardiac #dysfunction in a #cardiomyocyte-restricted mouse model of #SARS-CoV-2 infection, J Virol.: https://journals.asm.org/doi/full/10.1128/jvi.01179-24?af=R
These findings establish a mouse #model of SARS-CoV-2 cardiomyocyte infection that recapitulates features of cardiac dysfunctions of COVID-19 and suggests that both viral replication and resultant innate immune responses contribute to cardiac pathology.
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Read the full paper here: https://www.embopress.org/doi/full/10.1038/s44321-024-00093-3
" The insights derived from this analysis do not only support a CD19 CAR T cell-mediated reset of the memory B cell compartment, but also the parallel inhibition of the interferon signature
in #monocytes and #Tcells of #SLE patients"
https://insight.jci.org/articles/view/179433
Striking that this apparently works better in getting rid of certain memory #Bcells than anti-CD20 antibodies.
insight.jci.org
JCI Insight -
Selective CAR-T cell mediated B cell depletion suppresses interferon signature in SLE
#Hypoxia activates SREBP2 through Golgi disassembly in bone marrow-derived #monocytes for enhanced tumor growth
Tsuyoshi Osawa et al show that low-oxygen conditions induce lineage-specific activation of #SREBP2 via #Golgi-ER fusion, promoting lipid biosynthesis, myeloid cell infiltration, and #tumorigenesis
https://www.embopress.org/doi/full/10.15252/embj.2023114032
Very exciting that this paper is out now in #cancercell: https://www.cell.com/cancer-cell/fulltext/S1535-6108(23)00216-7
In brief: we show that in esophageal #adenocarcinoma #checkpoint #therapy success is predicted by an independent combination of mutation burden and the amount of #monocytes in the tumour pre-treatment (as measured by RNAseq). Very exciting link between #myeloid cells and #immune response to #cancer!
"NB cells signal to the BM microenvironment, rewiring via macrophage migration inhibitory factor and #midkine signaling specifically #monocytes ..."
#Neuroblastoma paper by Fetahu et al., the latest publication from the Taschner-Mandl lab in Vienna.
Going through this right now - very alike our own work - and talking to a few team members on Wednesday while they visit our center.
#macrophages #scATACseq #scRNAseq #OpenAccess #cancer #immunology
Data available on EGA, behind a DAC.
@LangzeitC This article is a perfect example of why #covid media coverage is part of the problem.
Article:
197! differences in the #epigenetics of #monocytes from #LongCovid patients! Whoot? 
Reading the actual paper, which can be found here:
https://clinicalepigeneticsjournal.biomedcentral.com/articles/10.1186/s13148-022-01398-1#MOESM1
you will find it is based on 10(!) patients and that there is an arbitrarily chosen difference of #methylation of 0.2 that leads to that number of 197. What that means? Who knows? But its enough to ventilate it..
BioMed CentralDefining post-acute COVID-19 syndrome (PACS) by an epigenetic biosignature in peripheral blood mononuclear cells - Clinical EpigeneticsPost-acute COVID-19 syndrome (PACS) has been defined as symptoms persisting after clearance of a COVID-19 infection. We have previously demonstrated that alterations in DNA methylation (DNAm) status persist in individuals who recovered from a COVID-19 infection, but it is currently unknown if PACS is associated with epigenetic changes. We compared DNAm patterns in patients with PACS with those in controls and in healthy COVID-19 convalescents and found a unique DNAm signature in PACS patients. This signature unravelled modified pathways that regulate angiotensin II and muscarinic receptor signalling and protein–protein interaction networks that have bearings on vesicle formation and mitochondrial function.
Second great thread from @jeffgilchrist explaining ways Covid gets around our immune system’s. Here he describes how it shifts monocytes from their protective function to one that leads to more clotting. And, the virus basically grows your nasal cilia like jack and the bean stock (his analogy) to bypass protective mucus.
Fascinating and gnarly.
mstdn.scienceJeff Gilchrist (@jeffgilchrist@mstdn.science)Attached: 1 image
How COVID actively suppresses and evades your immune system [Part 2]
Part 2 looks at how mild and moderate #COVID infection reprograms white #blood cells (#monocytes) to reduce #innate #immune functions and how it evades the mucus layer in our #nose 🧵 1/
The one page web view is also available here: https://pingthread.com/thread/1612066649934290946
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The paper focuses on #DendriticCells (DC) and #monocytes from a local inflammatory site, in this case synovial fluid from swollen joints of mainly juvenile #arthritis patients.
Very short: one type of DC were very much enriched but seemed relatively quiescent despite the inflammatory surroundings, while other DC and monocytes behaved clearly pro-inflammatory.
Last week, the paper based on my work in the Wilhelmina Children's Hospital (WKZ) was published, with the title "Conventional dendritic cells type 1 are strongly enriched, quiescent and relatively tolerogenic in local inflammatory arthritis".
https://doi.org/10.3389/fimmu.2022.1101999
Credits to Anoushka Samat for taking care of this after I left the department. Without her this work would not be out there. And of course thanks to the rest of the team.
FrontiersConventional dendritic cells type 1 are strongly enriched, quiescent and relatively tolerogenic in local inflammatory arthritisIntroductionDendritic cells (DC) are crucial for initiating and shaping immune responses. So far, little is known about the functional specialization of human DC subsets in (local) inflammatory conditions. We profiled conventional (c)DC1, cDC2 and monocytes based on phenotype, transcriptome and function from a local inflammatory site, namely synovial fluid (SF) from patients suffering from a chronic inflammatory condition, Juvenile Idiopathic Arthritis (JIA) as well as patients with rheumatoid arthritis (RA).MethodsPaired PB and SF samples from 32 JIA and 4 RA patients were collected for mononuclear cell isolation. Flow cytometry was done for definition of antigen presenting cell (APC) subsets. Cell sorting was done on the FACSAria II or III. RNA sequencing was done on SF APC subsets. Proliferation assays were done on co-cultures after CD3 magnetic activated cell sorting (MACS). APC Toll-like receptor (TLR) stimulation was done using Pam3CSK4, Poly(I:C), LPS, CpG-A and R848. Cytokine production was measured by Luminex.ResultscDC1, a relatively small DC subset in blood, are strongly enriched in SF, and showed a quiescent immune signature without a clear inflammatory profile, low expression of pathogen recognition receptors (PRRs), chemokine and cytokine receptors, and poor induction of T cell proliferation and cytokine production, but selective production of IFNλ upon polyinosinic:polycytidylic acid exposure. In stark contrast, cDC2 and monocytes from the same environment, ...
Great thread by Dr Gilchrist @jeffgilchrist
How COVID actively suppresses and evades your immune system
“ how mild and moderate #COVID infection reprograms white #blood cells (#monocytes) to reduce #innate #immune functions and how it evades the mucus layer in our #nose”
PingThreadThread by jeffgilchrist: How COVID actively suppresses and evades your immu... - PingThreadHow COVID actively suppresses and evades your immune system [Part 2]
Part 2 looks at how mild and moderate #COVID infection reprograms white #blood cells (#monocytes) to reduce #innate #immune functions and how it evades the mucus layer in our #nose. 🧵1/
How COVID actively suppresses and evades your immune system [Part 2]
Part 2 looks at how mild and moderate #COVID infection reprograms white #blood cells (#monocytes) to reduce #innate #immune functions and how it evades the mucus layer in our #nose 
1/
The one page web view is also available here: https://pingthread.com/thread/1612066649934290946
How #COVID actively suppresses and evades your #immune system [Part 1]
This thread highlights multiple methods that the virus uses to help keep #reinfecting people including #MHC-I and #interferon #suppression, #evasion of Natural Killer (NK) cells, and reprogramming of #monocytes. 1/
The one page web view is available here: https://pingthread.com/thread/1611333222419566594
Did you know that during #embryo development, migrating #monocytes deposit tracks of pro-angiogenic #migrasomes guiding the formation of blood vessels?
In this interesting manuscript published in Nature Cell Biology, researchers at Tsinghua University in Beijing studied this phenomenon in the chorioallantoic membrane of chick embryos: https://www.nature.com/articles/s41556-022-01026-3
This could have implications in organotypic #tissueengineering and provide new ways how to inhibit #angiogenesis in solid #tumors.
My first #toot and a #introduction
Currently in my 2nd year of a PhD studying #immunometabolism in #Monocytes and #macrophages in #Inflammation as a wannabe #clinicianscientist Also full-time servant to a mini panther (Oscar) and currently trying to navigate my way around Mastodon! Looking forward to meeting more people here!