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#WBE

0 posts0 participants0 posts today
Public *
William Perry

Check out this short write-up I put together, "Warmer samples for a cooler world", highlighting our recent paper.

The paper assessed the impact of different sample storage techniques on chemical (ammonium, orthophosphate, pH, conductivity, turbidity) and biological markers (SARS-CoV-2, CrAssphage) in #wastewater!

The big shock was that storage at bog-standard 4°C was a real winner:
cardiff.ac.uk/news/view/289539

Cardiff UniversityWarmer samples for a cooler worldBy storing wastewater samples at higher temperatures, laboratories can substantially decrease the amount of energy they use, and thus reduce their carbon emissions, without significantly compromising sample integrity.
#WBE#Research#MolecularEcology
Public
William Perry

Check out our newest paper on #wastewater sample storage best practice, published in Journal of Virological Methods: doi.org/10.1016/j.jviromet.2024.115063
What was really eye opening for me is that storing samples at 4C was often the best option for chemical and RNA markers, especially if it was for <30 weeks.
I also enjoyed exploring what switching from -80C to 4, or even -20, would do for CO2 saving, because -80 freezers are real energy guzzlers! #SARSCoV2 #WBE #SampleStorage #ClimateChange

Flow diagram showing the process implemented in this study which tested the impact of wastewater storage conditions on sample quality. Including collecting wastewater samples from a wastewater treatment works, transporting it to the laboratory, mixing the samples, storing the samples under different conditions, and then analysing those samples for different biological and chemical markers.
Public
William Perry

After a year of producing #COVID #wastewater reports back in 2022/23 for #Welsh #prisons, it's fantastic to see this paper out! doi.org/10.3389/fpubh.2024.146 Especially as we didn't have access to case data at the time.
V. convincing correlations between wastewater and traditional testing. I was also surprised by uninformed comments from Consultants in #CommunicableDisease Control.
For me, #WBE methods are clearly there but implementation needs improvement with better stakeholder engagement.

Wastewater processing pipeline from sample collection to report generation.
Confirmed positive COVID-19 cases (pink) and average SARS-CoV-2 wastewater concentration levels (gene copies per liter; blue) by week, 04/03/2022–24/10/2022.
Replied in thread
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NaraMoore ⛩️👻八尺様👻⛩️ at Fedi

#JIdol: Daily Tsukishiro Himari
第359🖤🐉🌙

／

✨🌙 Special Thanks!! ⚔✨

＼

International fans prepared a flower display and life-size panel of Persephone to celebrate Himari’s birthday at yesterday’s #WBE 🌹🎀

Thank you so much for your kindness!!💖

Image Source: Tsukishiro Himari

#月城ひまり #TsukishiroHimari #isiliel #Necronomidol #Necronama
#地下アイドル #アイドル #Idol #JMetal
#WBE #魔界 #Persephone #ペルセポネ

Himari dressed as Persephone stands next to a large flower stand.
Himari dressed as Persephone sits in the thinker position.
Replied in thread
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NaraMoore ⛩️👻八尺様👻⛩️ at Fedi

#JIdol: Daily Tsukishiro Himari
第358🖤🐉🌙

Birthday Message from Tsukishiro Himari:

My beloved…

There was a huge stand flower, a beautiful Persephonē illustration, and a life-size panel at the venue. I was so surprised… It was like a dream.

Today I received a lot of love in many ways. I’ll definitely come meet you and sing my love to you again!🌙♥︎

Image Source: Tsukishiro Himari

#月城ひまり #TsukishiroHimari #isiliel #Necronomidol #Necronama
#地下アイドル #アイドル #Idol #JMetal
#WBE #魔界 #Persephone #ペルセポネ

Hima is posed next to a large standee of herself. Behind them is a flower stand, moon ballon, and picture of Hima.
Replied in thread
Public
NaraMoore ⛩️👻八尺様👻⛩️ at Fedi

#JIdol: Daily Tsukishiro Himari
第355🖤🐉🌙

🌙
Tsukishiro Himari Birthday 2024 🌙

Himari’s birthday is on the same day as #WBE Ep. 2!

We’re preparing a flower stand and a surprise from the international fans to celebrate.

We’re accepting donations via PayPal at himaribirthday2023@gmail.com - will post pictures after the show!

Image source: Tsukishiro Himari

#月城ひまり #TsukishiroHimari #Isiliel #Necronomidol
#地下アイドル #アイドル #Idol
#JMetal #Metal #BlackMetal #BlackMetalMonday #Persephone

Poster for War of the Black Empirez EP 2. Shows Hima Drawing a katana.
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Denis - The COVID info guy -

Wastewater-based epidemiology (WBE) predicts COVID-19-induced weekly new hospital admissions in over 150 USA counties.

Although the coronavirus disease (COVID-19) emergency status is easing, the COVID-19 pandemic continues to affect healthcare systems globally. It is crucial to have a reliable and population-wide prediction tool for estimating COVID-19-induced hospital admissions.

#COVID19 @auscovid19 #WastewaterBasedEpidemiology #WBE

Source: nature.com/articles/s41467-023

NatureWastewater-based epidemiology predicts COVID-19-induced weekly new hospital admissions in over 150 USA counties - Nature CommunicationsWastewater-based epidemiology is increasingly used to predict disease occurrence. Here, the authors use SARS-CoV-2 RNA concentrations in wastewater in machine learning models to predict COVID-19 related hospitalisation in the United States.
Public
kath m oreilly

Reflections on the Testing the Water conference yesterday: learnt a lot about drugs testing in , I really liked the proposition of reporting ‘doses per 1,000 per day’ because it makes the data more relevant to public health. Inferring transient population size from metabolites 👍 but development still required. And great to catch up & meet new colleagues

Public
kath m oreilly

My new favourite paper: Online dashboards for SARS-CoV-2 data need standard best practices: An environmental health communication agenda pubmed.ncbi.nlm.nih.gov/372549 great because it reviews commonalities and differences across . Going forward, consistent measures of virus, details on how measured & log10 toggle needed

PubMedOnline dashboards for SARS-CoV-2 wastewater data need standard best practices: An environmental health communication agenda - PubMedThe COVID-19 pandemic has highlighted the benefits of wastewater surveillance to supplement clinical data. Numerous online information dashboards have been rapidly, and typically independently, developed to communicate environmental surveillance data to public health officials and the public. In thi …
Public
kath m oreilly

A very nice paper on - syst review of non-covid pathogens. Insight: very polio heavy, few studies explore public health impact. medrxiv.org/content/10.1101/20 and now in AJE

medRxivWastewater surveillance for infectious disease: a systematic reviewWastewater surveillance of SARS-CoV-2 has shown to be a valuable source of information regarding SARS-CoV-2 transmission and COVID-19 cases. Though the method has been used for several decades to track other infectious diseases, there has not been a comprehensive review outlining all of the pathogens surveilled through wastewater. The aim of this study is to identify what infectious diseases have been previously studied via wastewater surveillance prior to the COVID-19 Pandemic and identify common characteristics between the studies, as well as identify current gaps in knowledge. Peer-reviewed articles published as of August 1, 2020 that examined wastewater for communicable and infectious human pathogens on 2 or more occasions were included in the study. Excluded from this list were all reviews and methods papers, single collection studies, and non-human pathogens. Infectious diseases and pathogens were identified in studies of wastewater surveillance, as well as themes of how wastewater surveillance and other measures of disease transmission were linked. This review did not include any numerical data from individual studies and thus no statistical analysis was done. 1005 articles were identified but only 100 were included in this review after applying the inclusion criteria. These studies came from 38 countries with concentration in certain countries including Italy, Israel, Brazil, Japan, and China. Twenty-five separate pathogen families were identified in the included studies, with the majority of studies examining pathogens from the family Picornaviridae, including polio and non-polio enteroviruses. Most studies of wastewater surveillance did not link what was found in the wastewater to other measures of disease transmission. Among those studies that did compare wastewater surveillance to other measures of disease transmission the value observed was dependent upon pathogen and varied by study. Wastewater surveillance has historically been used to assess water-borne and fecal-orally transmitted pathogens causing diarrheal disease. However, numerous other types of pathogens have been surveilled using wastewater and wastewater surveillance should be considered as a potential tool for many infectious diseases. Wastewater surveillance studies can be improved by incorporating other measures of disease transmission at the population-level including disease incidence and hospitalizations. ### Competing Interest Statement The authors have declared no competing interest. ### Funding Statement No external funding was received for this work. ### Author Declarations I confirm all relevant ethical guidelines have been followed, and any necessary IRB and/or ethics committee approvals have been obtained. Yes The details of the IRB/oversight body that provided approval or exemption for the research described are given below: IRB is not applicable - this is a systematic review. All necessary patient/participant consent has been obtained and the appropriate institutional forms have been archived. Yes I understand that all clinical trials and any other prospective interventional studies must be registered with an ICMJE-approved registry, such as ClinicalTrials.gov. I confirm that any such study reported in the manuscript has been registered and the trial registration ID is provided (note: if posting a prospective study registered retrospectively, please provide a statement in the trial ID field explaining why the study was not registered in advance). Yes I have followed all appropriate research reporting guidelines and uploaded the relevant EQUATOR Network research reporting checklist(s) and other pertinent material as supplementary files, if applicable. Yes Data in the article is available from the authors upon reasonable request.
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